GHK-Cu Reconstitution Calculator

GHK-Cu is the copper(II) complex of the tripeptide glycyl-histidyl-lysine. Its vials (25 to 100 mg) are much larger than most peptides, so the resulting concentrations are in the tens of mg/mL. At 401.9 g/mol a given mg/mL is also a far higher molar concentration than for a large peptide, and the calculator reports both. Enter the vial size and the volume of bacteriostatic water added to get the concentration, the amount in any measured volume, and how many aliquots a vial yields. For other compounds, use the general peptide calculator.

GHK-Cu calculator

—Concentration

—per 0.1 mL (100 µL)

—molar concentration (MW 401.9 g/mol)

This tool does solution-concentration arithmetic for laboratory preparation records. It does not provide dosage guidance. GHK-Cu is sold for research use only. It is not for human consumption, self-administration or therapeutic use.

GHK-Cu vial concentration table

Worked math for each GHK-Cu vial size in the catalog (25 mg, 50 mg, 100 mg) with 1, 2 and 3 mL of diluent. The figures come straight from concentration = mass ÷ volume. They are reference arithmetic, not a recommended preparation.

Vial	Diluent	Concentration	Per 0.1 mL	Molarity
GHK-Cu 25 mg	1 mL	25 mg/mL	2,500 mcg	62.2 mM	Load ↑
GHK-Cu 25 mg	2 mL	12.5 mg/mL	1,250 mcg	31.1 mM	Load ↑
GHK-Cu 25 mg	3 mL	8.333 mg/mL	833.3 mcg	20.73 mM	Load ↑
GHK-Cu 50 mg	1 mL	50 mg/mL	5,000 mcg	124.41 mM	Load ↑
GHK-Cu 50 mg	2 mL	25 mg/mL	2,500 mcg	62.2 mM	Load ↑
GHK-Cu 50 mg	3 mL	16.667 mg/mL	1,666.7 mcg	41.47 mM	Load ↑
GHK-Cu 100 mg	1 mL	100 mg/mL	10,000 mcg	248.82 mM	Load ↑
GHK-Cu 100 mg	2 mL	50 mg/mL	5,000 mcg	124.41 mM	Load ↑
GHK-Cu 100 mg	3 mL	33.333 mg/mL	3,333.3 mcg	82.94 mM	Load ↑

GHK-Cu compound data used in the math

Compound class	Copper(II) complex of the tripeptide Gly-His-Lys
Molecular weight	401.9 g/mol
Molecular formula	C₁₄H₂₂CuN₆O₄
CAS number	89030-95-5
Appearance	Blue to blue-violet lyophilized powder; distinctly blue solution
Storage	Lyophilized: −20 °C, desiccated, protected from light and moisture. Reconstituted: 2–8 °C.
Vial sizes	25 mg, 50 mg, 100 mg

Molar concentration (mM) = concentration (mg/mL) ÷ molecular weight (g/mol) × 1000. Every product ships with a certificate of analysis. See the GHK-Cu product page or the COA portal.

GHK-Cu reconstitution handling notes

The solution should be clearly blue, and the color comes from the copper complex. It gets deeper as concentration rises. A colorless solution, a brown tint or a precipitate means the lot’s certificate of analysis should be checked before use.
With 50 mg and 100 mg vials, the mass-per-aliquot figure is usually in milligrams rather than micrograms. Enter it in mcg here (1 mg = 1000 mcg).
Because of the low molecular weight, 10 mg/mL of GHK-Cu is about 24.9 mM. That is roughly ten times the molarity of a 10 mg/mL solution of a 4–5 kDa peptide (about 2–2.5 mM).

For the full bench procedure, including materials, technique, blend vials and storage before and after, see how to reconstitute peptides.

How to reconstitute GHK-Cu

The worked example uses a 50 mg GHK-Cu vial and 2 mL of bacteriostatic water. The same steps apply to any vial size or volume; only the resulting concentration changes.

Equilibrate and sanitize. Let the GHK-Cu vial and the bacteriostatic water reach room temperature, then wipe both rubber stoppers with an alcohol swab.
Draw the diluent. With a sterile syringe, withdraw 2 mL of bacteriostatic water.
Add it slowly down the wall. Insert the needle through the GHK-Cu vial’s stopper and let the water run down the inside of the glass, not directly onto the powder.
Swirl, don’t shake. Rotate the vial gently until the solution is clear and uniformly blue. Cloudiness or particles after gentle mixing are a reason to check the lot’s certificate of analysis.
Label and store. Write the concentration on the vial: 50 mg ÷ 2 mL = 25 mg/mL (62.2 mM), plus the date and lot number. Storage: Lyophilized: −20 °C, desiccated, protected from light and moisture. Reconstituted: 2–8 °C.

GHK-Cu reconstitution FAQ

What concentration does a 25 mg GHK-Cu vial make with 2 mL of bacteriostatic water?

25 mg divided by 2 mL is 12.5 mg/mL, which is 1,250 mcg in every 0.1 mL. Using GHK-Cu’s molecular weight of 401.9 g/mol, that is a molar concentration of 31.1 mM.

How much GHK-Cu is in 0.1 mL after reconstitution?

It depends entirely on the concentration prepared. For example, a 50 mg vial reconstituted with 2 mL gives 25 mg/mL, so 0.1 mL contains 2,500 mcg. The same vial reconstituted with 1 mL would give twice that per 0.1 mL.

Does adding more bacteriostatic water change how much GHK-Cu is in the vial?

No. The diluent volume only sets the concentration. The total GHK-Cu in the vial is the same whatever volume is added. More diluent spreads it across a larger volume, so any given amount takes up more mL and is easier to measure accurately.

How should reconstituted GHK-Cu be stored?

Lyophilized: -20 °C, desiccated, protected from light and moisture. Reconstituted: 2-8 °C. Label the vial with the compound, lot number, date and the concentration calculated here.