Tirzepatide Reconstitution Calculator

Tirzepatide is a 39-amino-acid peptide with a C20 fatty diacid side chain, supplied as a lyophilized powder. At about 4,813 g/mol it is heavier than semaglutide, so the same mg/mL gives a lower molar concentration. This calculator reports both figures. Enter the vial size and the volume of bacteriostatic water added to get the concentration, the amount in any measured volume, and how many aliquots a vial yields. For other compounds, use the general peptide calculator.

Tirzepatide calculator

—Concentration

—per 0.1 mL (100 µL)

—molar concentration (MW 4,813.5 g/mol)

This tool does solution-concentration arithmetic for laboratory preparation records. It does not provide dosage guidance. Tirzepatide is sold for research use only. It is not for human consumption, self-administration or therapeutic use.

Tirzepatide vial concentration table

Worked math for each Tirzepatide vial size in the catalog (5 mg, 10 mg, 20 mg) with 1, 2 and 3 mL of diluent. The figures come straight from concentration = mass ÷ volume. They are reference arithmetic, not a recommended preparation.

Vial	Diluent	Concentration	Per 0.1 mL	Molarity
Tirzepatide 5 mg	1 mL	5 mg/mL	500 mcg	1.04 mM	Load ↑
Tirzepatide 5 mg	2 mL	2.5 mg/mL	250 mcg	519 µM	Load ↑
Tirzepatide 5 mg	3 mL	1.667 mg/mL	166.7 mcg	346 µM	Load ↑
Tirzepatide 10 mg	1 mL	10 mg/mL	1,000 mcg	2.08 mM	Load ↑
Tirzepatide 10 mg	2 mL	5 mg/mL	500 mcg	1.04 mM	Load ↑
Tirzepatide 10 mg	3 mL	3.333 mg/mL	333.3 mcg	692 µM	Load ↑
Tirzepatide 20 mg	1 mL	20 mg/mL	2,000 mcg	4.15 mM	Load ↑
Tirzepatide 20 mg	2 mL	10 mg/mL	1,000 mcg	2.08 mM	Load ↑
Tirzepatide 20 mg	3 mL	6.667 mg/mL	666.7 mcg	1.38 mM	Load ↑

Tirzepatide compound data used in the math

Compound class	39-amino-acid acylated dual GIP / GLP-1 receptor agonist
Molecular weight	4,813.5 g/mol
Molecular formula	C₂₂₅H₃₄₈N₄₈O₆₈
CAS number	2023788-19-2
Appearance	White to off-white lyophilized powder; clear, colorless solution
Storage	Lyophilized: −20 °C, desiccated, protected from light. Reconstituted: 2–8 °C.
Vial sizes	5 mg, 10 mg, 20 mg

Molar concentration (mM) = concentration (mg/mL) ÷ molecular weight (g/mol) × 1000. Every product ships with a certificate of analysis. See the Tirzepatide product page or the COA portal.

Tirzepatide reconstitution handling notes

Because tirzepatide’s molecular weight is about 17% higher than semaglutide’s, a 5 mg/mL tirzepatide solution is roughly 1.04 mM, while semaglutide at the same mass concentration is about 1.22 mM. Comparative studies set up by mass alone are not equimolar.
The larger (20 mg) vial reconstituted in a small volume gives a concentrated solution. If aliquots come out below about 0.02 mL, reconstitute with more diluent so they are easier to measure accurately.
Record the lot number and the concentration reported here on the vial label. The certificate of analysis for that product holds the measured purity.

For the full bench procedure, including materials, technique, blend vials and storage before and after, see how to reconstitute peptides.

How to reconstitute Tirzepatide

The worked example uses a 10 mg Tirzepatide vial and 2 mL of bacteriostatic water. The same steps apply to any vial size or volume; only the resulting concentration changes.

Equilibrate and sanitize. Let the Tirzepatide vial and the bacteriostatic water reach room temperature, then wipe both rubber stoppers with an alcohol swab.
Draw the diluent. With a sterile syringe, withdraw 2 mL of bacteriostatic water.
Add it slowly down the wall. Insert the needle through the Tirzepatide vial’s stopper and let the water run down the inside of the glass, not directly onto the powder.
Swirl, don’t shake. Rotate the vial gently until the solution is completely clear and colorless. Cloudiness or particles after gentle mixing are a reason to check the lot’s certificate of analysis.
Label and store. Write the concentration on the vial: 10 mg ÷ 2 mL = 5 mg/mL (1.04 mM), plus the date and lot number. Storage: Lyophilized: −20 °C, desiccated, protected from light. Reconstituted: 2–8 °C.

Tirzepatide reconstitution FAQ

What concentration does a 5 mg Tirzepatide vial make with 2 mL of bacteriostatic water?

5 mg divided by 2 mL is 2.5 mg/mL, which is 250 mcg in every 0.1 mL. Using Tirzepatide’s molecular weight of 4,813.5 g/mol, that is a molar concentration of 519 µM.

How much Tirzepatide is in 0.1 mL after reconstitution?

It depends entirely on the concentration prepared. For example, a 10 mg vial reconstituted with 2 mL gives 5 mg/mL, so 0.1 mL contains 500 mcg. The same vial reconstituted with 1 mL would give twice that per 0.1 mL.

Does adding more bacteriostatic water change how much Tirzepatide is in the vial?

No. The diluent volume only sets the concentration. The total Tirzepatide in the vial is the same whatever volume is added. More diluent spreads it across a larger volume, so any given amount takes up more mL and is easier to measure accurately.

How should reconstituted Tirzepatide be stored?

Lyophilized: -20 °C, desiccated, protected from light. Reconstituted: 2-8 °C. Label the vial with the compound, lot number, date and the concentration calculated here.